Detection on the Promotion Effect on Angiogenic and Osteogenic Capabilities of the Necrosis Parts from Ad-BMP-2-IRES-HIF-1α After Transfecting AdBMP-2-IRES-HIF-1αto the Induced Rabbit Bone Marrow Derived Endothelial Progenitor Cells(EPCs) and then Implant
Purpose To discuss the clinical effect of locally treating the parts of necrosis of the femoral head by means of transplanting Ad-BMP-2-IRES-HIF-1αto the endothelial progenitor cells(EPCs) and then implanting it into the necrosis parts of the femoralhead.MethodsTheAd-BMP-2- IRES-HIF-1α was transfected into induced EPCs and then transplanted into avascular necrotic parts of the femoral head(ANFH), and then, the promotion effect on angiogenic and osteogenic capabilities of the necrosis parts from Ad-BMP-2-IRES-HIF-1α was detected. Rabbit bone marrow MNCs were obtained by density gradient centrifugation method, and were induced into EPCs by M199 medium; EPCs were identified in accordance with the cell morphology, specific surface markers and uptake abilities. The Ad-BMP-2-IRES-HIF-1α was transfected to EPCs and then transplanted into parts of ANFH. The models were enthanized2 and 4 weeks after operation, and then the angiogenic and osteogenic indexes of necrotic parts were detected. Results The results showed that more blood vessels generated in group A than that in group B and C (P<0.05), and the statistical differences were found between group B and C(P<0.05); The detection of histology andBMP-2 immuno histochemistry showed that there were statistical differences between group A and B, group A and C (P<0.05). There was no statistical difference between group B and C (P>0.05). Conclusion To sum up, this experiment shows that the EPCs transfected by Ad-BMP-2-IRES-HIF-1α have stronger angiogenic and osteogenic capabilities.