Detection on the Promotion Effect on Angiogenic and Osteogenic Capabilities of the Necrosis Parts from Ad-BMP-2-IRES-HIF-1α After Transfecting AdBMP-2-IRES-HIF-1αto the Induced Rabbit Bone Marrow Derived Endothelial Progenitor Cells(EPCs) and then Implant

  • Fuai Cui School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China
  • Xiuli Wang the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Wei Wang School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China
  • Peng Xiao School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China
  • Yuan Ma the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Lindong Jiang the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Keywords: Ad-BMP-2-IRES-HIF-1α, Ad-CMV-IRES-hrGFP-1, EPCs, avascular necrosis of the femoral head, angiogenesis, BMP-2

Abstract

Purpose To discuss the clinical effect of locally treating the parts of necrosis of the femoral head by means of transplanting Ad-BMP-2-IRES-HIF-1αto the endothelial progenitor cells(EPCs) and then implanting it into the necrosis parts of the femoralhead.MethodsTheAd-BMP-2- IRES-HIF-1α was transfected into induced EPCs and then transplanted into avascular necrotic parts of the femoral head(ANFH), and then, the promotion effect on angiogenic and osteogenic capabilities of the necrosis parts from Ad-BMP-2-IRES-HIF-1α was detected. Rabbit bone marrow MNCs were obtained by density gradient centrifugation method, and were induced into EPCs by M199 medium; EPCs were identified in accordance with the cell morphology, specific surface markers and uptake abilities. The Ad-BMP-2-IRES-HIF-1α was transfected to EPCs and then transplanted into parts of ANFH. The models were enthanized2 and 4 weeks after operation, and then the angiogenic and osteogenic indexes of necrotic parts were detected. Results The results showed that more blood vessels generated in group A than that in group B and C (P<0.05), and the statistical differences were found between group B and C(P<0.05); The detection of histology andBMP-2 immuno histochemistry showed that there were statistical differences between group A and B, group A and C (P<0.05). There was no statistical difference between group B and C (P>0.05). Conclusion To sum up, this experiment shows that the EPCs transfected by Ad-BMP-2-IRES-HIF-1α have stronger angiogenic and osteogenic capabilities.

Author Biographies

Fuai Cui, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China

Institute of Biochemistry and Molecular Biology,

Xiuli Wang, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China

Department of Orthopaedic Surgery, 

Wei Wang, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China

Institute of Biochemistry and Molecular Biology, 

Peng Xiao, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong 250012, P.R. China

Institute of Biochemistry and Molecular Biology, 

Yuan Ma, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China

Department of Orthopaedic Surgery,

Lindong Jiang, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China

Department of Orthopaedic Surgery, 

Published
2016-06-05