UV Method Development and Validation of Eperisone Hydrochloride in Bulk and Tablet Formulation

Saima Saleem; Saeeda Bano; Safila Naveed; Huma Dilshad; M. Tanweer Alam; Amanullah Khan; Ghulam Sarwar

Saima Saleem Jinnah University for Women, Karachi Pakistan.
Saeeda Bano Plattinum Pharmaceuticals (pvt) ltd
Safila Naveed Jinnah University for Women, Karachi
Huma Dilshad Jinnah University for Women, Karachi Pakistan.
M. Tanweer Alam Jinnah University for Women, Karachi Pakistan.
Amanullah Khan Health Department Baluchistan
Ghulam Sarwar Jinnah University for Women, Karachi Pakistan.

Keywords: Method Validation, Eperisone Hydrochloride, UV Spectromphotometer

Abstract

Objective: To develop and validate a UV simple, precise and cost saving method for fast analysis of Eperisone Hydrochloride in Eperisone tablets.

Method: The test is developed by using methanol as diluent, performed on UV visible double beam spectrophotometer at range of 200nm-400nm, and found absorbance on maximum wavelength of 260 nm. The test is then validated by using complete parameters of method validation i.e., linearity, precision, selectivity, accuracy, repeatability, robustness, limit of quantitation and detection. The complete procedure is carried out as per United State Pharmacopeia, ICH and WHO guidelines.

Results: Calculate individually all parameters of method validation as per standard that should meet the validation criteria.

Conclusion: As per above discussion the method is precise and accurate for analysis of Eperisone hydrochloride in Eperisone hydrochloride tablets.