Mebendazole Inexplicably Reducing the Breast Cancer Cells Viability Preclinically by Incitement Effects with Methotrexate

  • Dow International Medical College
  • Dow University of Health Sciences, Karachi, Pakistan
  • Dow University of Health Sciences, Karachi, Pakistan
  • Dow University of Health Sciences, Karachi, Pakistan
Keywords: MCF-7, MDA-MB-231, MTT, Trypan blue dye exclusion assay, Mebendazole

Abstract

Objective: To perform preclinical (in vitro) interventional trial of mebendazole.
Methods: This study carried out in Basic Medical Science Institute, JMPC in alliance with PCMD. The total study duration from March 2016 to February 2017. For assessment of cytotoxic incitement effects of mebendazole with methotrexate we used six different dilutions of mebendazole (1.5µM - 100µM) both as alone and in combination with methotrexate (0.5µM-100µM). Cytotoxicity was assessed by MTT and trypan blue dye exclusion cytotoxicity assays. We used primarily two breast cancerous cell lines MCF-7 (representative of invasive ductal carcinoma) and MDA-MB-231 (representative of adenocarcinoma).
Results: Mebendazole more effectively reducing the % viability of studied cancerous cell lines as combination therapy with methotrexate. The average percentage decrease of % viability of MCF-7 and MDA-MB-231 were -81.3097 and -66.8711 respectively. This combination showed selectivity towards cancerous cell lines as indicated by non-significant (p=0.183) effects on normal breast epithelial cell line (MCF-10).
Conclusion: This study demonstrated that combination therapy of mebendazole with standard chemotherapeutic agent methotrexate unveil incitement effects.

Author Biographies

, Dow University of Health Sciences, Karachi, Pakistan

Dow International Medical College, 

, Dow University of Health Sciences, Karachi, Pakistan

Dow International Medical College, 

, Dow University of Health Sciences, Karachi, Pakistan

Dow International Medical College, 

Published
2018-09-25
Section
Articles