Comparative Acute Oral Toxicity Study of Verona Anti-BPH with Verona Max in Mice

  • Hina Rehman Jinnah Sindh Medical University, Karachi, Pakistan
  • Saira Bano Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan
  • Faheem Akhtar Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan
  • Saima Asif Jinnah University for Women, Karachi, Pakistan
  • Aqib Zahoor Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan
  • Kashifa Khanum Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan
  • Safila Naveed Jinnah University for Women, Karachi, Pakistan
  • Khan Usmanghani Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan
Keywords: Benign Prostate Hyperplasia (BPH), verona, acute oral toxicity, herbal remedy

Abstract

Objective: Benign Prostate hyperplasia (BPH) is hyperplasia of prostate gland and age related diseases in men. 20%-60% men have BPH with the age of 40 to 60 years. The cause is not very well researched however the pharmacological and surgical current treatments have numerous toxic effects on quality of life. Natural medicines are known for its safety as compare to pharmacological choice of medicines. By considering this fact, current experimental study was established to compare and assess the acute oral toxicity of the Verona Anti-PBH with Verona Max in NMRI strain of mice on animal model.

Methodology: NMRI mice were obtained for experimental purpose from animal house facility of Herbion Pvt. Ltd. Constant environmental conditions were 25 ± 1 °C with R.H 52 - 61% with 12 hours dark and light cycle. As per desire, food and water were available. Animals were divided into three different groups including two treatment and control group. Treatment group including Verona Anti-BPH and Verona Max however control group were on water. Animal’s age were 6-8 weeks and average body weight were 25-35 g however cage cards were used for identifications between the groups. Mice were treated orally using gastric gavage with doses (1 or 5g/kg) of test product for 14 days and observed daily.

Results: The Verona anti-BPH and Verona Max did not cause any sign of ill health and acute toxicity in mice at the given doses of 1 or 5 g/kg. No hair loss, color loss, heart rate (chest expansion and retraction), writhing, stretching and fecal abnormality was observed. No remarkable change in the behavior and no mortality was observed.

Conclusion: The present study evaluated that Verona anti-BPH and Verona Max extract is potentially safe and no acute oral toxicity has found.

Author Biographies

Hina Rehman, Jinnah Sindh Medical University, Karachi, Pakistan

Faculty of Pharmacy, 

Saira Bano, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Research & Development, 

Faheem Akhtar, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Research & Development, 

Saima Asif, Jinnah University for Women, Karachi, Pakistan

Faculty of Pharmacy,

Aqib Zahoor, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Research & Development, 

Kashifa Khanum, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Research & Development, 

Safila Naveed, Jinnah University for Women, Karachi, Pakistan

Faculty of Pharmacy, 

Khan Usmanghani, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Research & Development, 

Published
2018-12-31