Quantitative HPLC Analysis of Vitamin D3 and Gallic Acid in Vivabon syrupfor Children Growth
Abstract
Objective:The current study was conducted to evaluate quantitative estimation of the biomarkers gallic acid and Vitamin D3 present in Vivabon-D3 syrup
Backgrounds:Vivabon-Vitamin D3 is a poly herbal syrup formulation for the promotion and growth of childrens. Vivabon comprises of Phoenix sylvestris, Phyllanthus emblica (synonym Emblica officinalis), Centella asiatica, Withania somnifera, Salvia haematodes, Centaurea behen, Zingiber officinalis, Trigonella foenum-greacum, Piper longum, Ammomum subulatum.
Methods:The present work has been carried out on polyherbal formulation for the quantitative determination of Vitamin D3 and gallic acid as markers.
Results:. Quantitative analysis of gallic acid was conducted; using silica gel 60 F254 coated plates as a stationary phase to augment the identification and determination of gallic acid components.
Conclusion: A rapid method for the quantitation of Vitamin D3 present in the Vivabon Vitamin D3syrup has been developed and validated in terms of linearity, precision, repeatability, limit of detection, limit of quantification and accuracy. The study result revealed that vivabon-D3syrup formulation was well standardized for routine quality control procedure.
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