Marker Based Standardization of Polyherbal Formulation Bonjigar by Spectrophotometric Method
Background:Bonjigar is an herbal oralpreprationhaving multiple ingridents with the effect of liver protection including. Glycyrrhiza glabra, Eclipta elba, Tamrix gallica, Sliymum marianum, Sphaeranthus indicus, Boerhaavia diffusa and Cichorium intybus. They have hepatoprotective,anti inflammatory, antioxidant, antihyperlipidemic and antipyreticetc activities which could be used as a potential source of bioactive substances.
Objectives:The objective of the study is to standardize Bonjigar using flavones, tannins and sapogenin as chemical markers with the aid of spectroscopy.
Methodology: Quantitative determination of flavones, sapogenins and phenolic compounds was evaluated at 360nm, 430nm and 277nm. Sample was prepared for the quantitative determination of each marker and was determined with a spectrophotometer.
Result:Evidently found that Bonjigar individually consist of flavanoids, tannins and sapogenins. Total flavanoids as rutin, tannins as gallic acid and total sapogenins as diosgenin were found to be 0.564 mg/gm, 14.30 mg/gm and 2.839 mg/gm respectively
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