Comparative Analysis of Antibacterial Activity of Ciprofloxacin and Homeopathic Mother Tincture

Abstract

Objective: The aim of this study was to evaluate and compare in vitro antibacterial effects of ciprofloxacin and homeopathic mother tinctures.
Methods: All the experimental homeopathic mother tinctures were donated by Masood Homeopathic Pharmaceuticals, Pakistan. Among tested bacteria, Escherichia coli (014) and Bacillus subtilis (143) were taken in the form of standard culture agar from the First Fungal Culture Bank of Pakistan (FCBP), Institute of Agricultural Sciences (IAGS), the University of Punjab, Lahore and Pseudomonas aeruginosa (ATCC 9027) and Staphylococcus aureus (ATCC 6538) were taken from American Type Culture Collection (ATCC). Ciprofloxacin (standard antibiotic) 1mg/ml was taken as positive control and 70% alcohol was taken as negative control. Homeopathic mother tinctures were checked for their antibacterial activity against four bacterial strains (Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) through agar well diffusion method.
Results: Ciprofloxacin expressed maximum activity against Pseudomonas aeruginosa among all tested bacteria. All tested mother tinctures expressed more or less antibacterial activity against Staphylococcus aureus with maximum activity of Eucalyptus globulus (zone of inhibition 21.7 mm). Eight out of ten mother tinctures expressed activity against Escherichia coli with maximum activity of Eucalyptus globulus (zone of inhibition 17 mm). Nine out of ten homeopathic mother tinctures expressed significant antibacterial activity against Pseudomonas aeruginosa with maximum activity of Hydrastis canadensis (zone of inhibition 28 mm). Similarly, nine out of ten homeopathic mother tinctures expressed significant antibacterial activity against Bacillus subtilis with maximum activity of Hydrastis canadensis (zone of inhibition 26 mm).
Conclusion: Hence, concluded that homeopathic mother tinctures showed antibacterial potential.