Acute and Sub Chronic Oral Toxicity Study of Linkus (EMA), Plantain Syrup and AltheaSH Tablet in Mice

Abstract

Objectives: The present preclinical investigation was conducted with the aim to acknowledge the safety of three polyherbal formulations Linkus syrup (EMA), Plantain syrup and AltheaSH tablet extract by evaluating the acute and sub chronic oral toxicity studies in mice.

Methods: To determine acute toxicity mice were given individually the doses of 1 or 5 g/kg orally of Linkus syrup (EMA), Plantain syrup and AltheaSH tablet extract. For executing sub chronic oral toxicity, the formulations were given in doses of 50, 100 and 200 mg/kg of body weight for 28 days period.

Results: All three polyherbal formulations under test were not found to be the reason of mortality in animals at the administered doses of 1 or 5 g/kg. Further toxicity signs including loss of hairs, lacrimation, weight reduction, gait, tremors and drowsiness were not detected.

Conclusion: The current investigation provided confirmation of good tolerance of formulations under test and lack of harmful effects on the functions of the vital body organs of mice in acute and sub chronic oral toxicity analysis. Future prospects will be the conduction of clinical trials of the formulations as the clinical efficiency is verified in preclinical studies.