Carbon Tetrachloride-Induced Hepatocellular Damage in Balb C Mice and Pharmacological Intervention by Extract of Daucus Carota

  • tafail akbar mughal government college university Lahore,
  • Shaukat Ali Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan
  • Ali Hassan Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan
  • Muhammad Zubair Saleem College of Basic Medical Sciences, Dalian Medical University, Dalian Liaoning, China
  • Shumaila Mumtaz Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan
  • Samaira Mumtaz Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan
Keywords: Hepatotoxicity, Carbon tetrachloride, Daucus carota root extract, Balb C mice, Pre-treatment.

Abstract

Background: Present research was planned to examine the shielding effect of Daucus carota root extract pre-treatment on the carbon tetrachloride-induced hepatotoxicity in Balb C mice.

Objective: To cover the assessment of the enzymatic activity such as Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) and biochemical constituents like cholesterol, protein, lipids, glucose and urea contents in both the blood and liver while RNA and DNA substances exclusively in the liver.

Setting: The induction of carbon tetrachloride (CCl4)-induced hepatotoxicity caused a rise in ALT of plasma and a decline in LDH.

Method: The mice were treated with CCl4 and Daucus carota extract to check their effect on the level of biochemical constituents.

Main Outcome Measure: Daucus carota extract pre-treatment obliterated CCl4-induced variations in the actions of enzymes.

Results: cholesterol content decreased while glucose level increased in the blood. Glucose, lipids, cholesterol and urea in the liver were reduced while total protein level rose. Daucus carota extract pre-treatment also prohibited CCl4-induced variations in total protein contents, urea, glucose and lipids in the liver. CCl4 action caused huge hepatic damage. This was averted by Daucus carota extract.

Conclusion: These outcomes reveal that Daucus carota root extract pre-treatment inhibited hepatic damage induced by CCl4 in the mice model, which corroborates its defensive effects against hepatic damage produced by both non-oxidative and oxidative mechanisms.

Published
2021-03-18