Screening of Ethanolic Extract for Antioxidant and anti-bacterial Activities of Some Selected Plants from Cholistan Desert

Abstract

Objective: Increase in the drug resistance and development of new strains is a source of discomfort for researchers working in developing countries. For restoration of life and health, it is essential to develop new pharmaceutical products that could replace the resistant antibiotics. Traditional medicine from Cholistan desert plays a novel role in this concern. Plant remedies and their formulations have been used extensively for the treatment of various diseases. Tinospora cordifolia (T. cordifolia) and Moringa olifera (M. olifera) possess different pharmacological activities. The aim of this study is to estimate the antioxidant and antibacterial screening of ethanolic extract of T. cordifolia and M. olifera.

Method: Antioxidant activity was done by DPPH method and anti-bacterial activity was done by agar disk diffusion and well diffusion method. Ciprofloxacin was used as the standard drug.

Results: Antioxidant activity indicates the % inhibition of 79.43345±0.16 and 89.15352±0.9 of T. cordifolia and M. olifera which is comparable with standard (Ascorbic acid) shows percent inhibition 93.74±0.14. Antibacterial activity was done with four gram negative bacterial strains and result was comparable with standard drug. In disk diffusion method ethanolic extract of M. olifera showed maximum zone of inhibition 30mm against Micrococcus Luteus and E coli, and T. cordifoli, and 20mm against Micrococcus Luteus. While in Well diffusion method M. olifera showed maximum zone of inhibition (19mm) against Bordetella bronchiseptica and T. cordifolia 17mm against Micrococcus Luteus. However, the observed zone of inhibition is less than standard Ciprofloxacin zone of inhibition.

Conclusion: It is concluded from the fact that T. cordifolia and M. olifera possess anti-bacterial potential against particular microorganism.