Analysis of Famotidine in API and Formulation using UV and HPLC

Abstract

Famotidine is Histamine-2 blocker. These H2 antagonists are competitor of histamine. Hydrochloric acid is secreted by the Parietal cells that are present in epithelial cells of stomach and stifle the secretion of acid. We have developed the Spectrophotometric technique for the analysis of Famotidine. A drug sample dissolved in water to make Famotidine solution. In the same way, different dilutions were made. Absorbance was measured at 265nm and the assay was performed at 5 different dilutions of 100ppm. Percent assay and regression equation was also obtained to calculate further availability of drug.
A simple, efficient and least time consuming method was validated by HPLC. Company Shimadzu LC 20AT model was used containing 150 mm column. Mobile phase having ratio of (80:20, v/v) methanol: water was used and pH was adjusted to 3.30. Analyte concentrations were determined at wavelength 265 nm using a UV-detector. All analyses were carrying out at room temperature (25 ± 2C). Famotidine was separated in formulations in aprox 5.0 min, linear calibration curves were obtained at concentration range from 100–25ppm. 0.9998 of correlation coefficients with an average recovery above 99.91% was also detected. Also retention time (Rt) recognition of analytes from different dosage forms shows the stability and specificity of the developed method.