Acute and Sub Chronic Oral Toxicity Studies of Weight Loss Formulation in Experimental Animal Models

Abstract

Objective: The current preclinical study aimed at appraising the claim of safety of the polyherbal weight reducing tablet formulation by evaluating its acute and sub chronic oral toxicity.

Methodology: The acute oral toxicity of weight loss tablets was evaluated as per the guidelines of Organization for Economic Co-operation and Development (OECD). Animals were divided into four groups (n=6) Group I, II and III were treated as test groups and received 7mg/kg (one tablet), 14mg/kg (2 tablets) and 21mg/kg (03 tablets) according to body weight respectively. Group IV received only distilled water and served as control group. An under test drug was administered at doses of 50, 100, 250 and 500 mg/kg as per the body weight for 28 days for determining sub chronic oral toxicity.

Result: Polyherbal weight reducing tablets formulation were not found to be the reason of any mortality in albino mice at the specified doses. Other signs of toxicity like hair loss, mucus membrane (nasal), lacrimation, drowsiness, gait and tremors were also not observed.

Conclusion: The present study gave evidence of good tolerance of formulation and the absence of detrimental effects on the functional state of the vital organs of the experimental animals in acute and sub chronic oral toxicity test. Future prospects include the clinical trials of the finished product as the clinical efficacy is proven in animal studies.