Evaluation of in vitro lipoxygenase Inhibition and Antioxidant Activity of Polyherbal Formulation Entoban

  • Sadia Shakeel Dow University of Health Sciences, Karachi, Pakistan.
  • Khan Usmanghani Herbion Pakistan (Pvt.) Ltd., Karachi, Pakistan
  • Allah Nawaz University of Toyama, Japan
  • Shahbaz Ahmad Zakki University of Toyama, Japan
  • Zheng-Guo Cui University of Toyama, Japan
  • Shugufta Abrahim Jazan University, Saudi Arabia
  • Tanveer Ahmad Mir University of Toyama, Japan

Abstract

Lipoxygenases are family of iron containing dioxygenases that convert the addition of molecular oxygen to fatty acid containing a cis-1, 4- pentadiene system. The primary product of this reaction is a 4-hydroperoxycis trans-1, 3-conjugated pentadienyl moiety within unsaturated fatty acid. This assay measures the hydroperoxides produced in the lipoxygenation reaction using a purified lipoxygenase with lionoleic acid as substrate. When formulations of Entoban syrup and capsules were compared at various concentrations (10, 50 and 100 µg/ml), lipoxygenase inhibition activity increased in a dose dependent manner for both formulations just like standard bacilein. The formulations under test have good potential of lipoxygenase inhibition and prospective to be used in the treatment of various complications produced by lipoxygenase enzymes.

Author Biographies

Allah Nawaz, University of Toyama, Japan

First Department of Internal Medicine, Faculty of Medicine, Graduate School of Medical and Pharmaceutical Sciences,

Shahbaz Ahmad Zakki, University of Toyama, Japan

Department of Public Health, Faculty of Medicine, Graduate School of Medical and Pharmaceutical Sciences,

Zheng-Guo Cui, University of Toyama, Japan

Department of Public Health, Faculty of Medicine, Graduate School of Medical and Pharmaceutical Sciences,

Shugufta Abrahim, Jazan University, Saudi Arabia

Department of computer Sciences and health Information systems,

Tanveer Ahmad Mir, University of Toyama, Japan

Department of Biomedical Engineering, Graduate School of Science and Engineering for Research,

Published
2015-12-12
Issue
Vol 3 No 2 (2015): Volume 3 2 2015
Section
Articles

Copyright (c) 2015 RADS Journal of Pharmacy and Pharmaceutical Sciences

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