Simultaneous Determination of Ibuprofen and Sitagliptin by HPLC
Ibuprofen is a non-steroidal anti-inflammatory drug (NSAID), widely used over the counter drug worldwide. Co-administration of antidiabetic drugs with non-steroidal anti-inflammatory drug (NSAID) such as Ibuprofen is quite common. Rapid liquid chromatographic procedure proposed for analysis of Ibuprofen and Sitagliptin in pharmaceutical preparations using methanol: water (80:20 v/v) as a mobile phase, adjusting pH to 3.2 with UV detection at 275 nm. The advantages of this method include good and rapid separation, well resolved peaks, and only a small amount of sample is required for assay that display adequate precision. The method showed good linearity in the range of 231.25-1000 μg mL−1 for Ibuprofen and Sitagliptin with a correlation coefficient of 0.9999. The recovery of Ibuprofen and Sitagliptin was > 99.99% and > 99.96%, respectively. The proposed method may be used for the quantitative analysis of Ibuprofen and sitagliptin alone or in combination in bulk drugs and combined dosage formulations.
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