Simultaneous Determination of Ibuprofen and Sitagliptin by HPLC

  • Hina Rehman Jinnah University for Women, Karachi
  • Safila Naveed Jinnah University for Women, Karachi
  • Fatima Qamar Jinnah University for Women, Karachi
  • Khan Usmanghani Jinnah University for Women, Karachi
  • Ghulam Sarwar Jinnah University for Women, Karachi
  • M. Tanweer Alam Jinnah University for Women, Karachi
  • Amanullah Khan Health Department Baluchistan

Abstract

Ibuprofen is a non-steroidal anti-inflammatory drug (NSAID), widely used over the counter drug worldwide. Co-administration of antidiabetic drugs with non-steroidal anti-inflammatory drug (NSAID) such as Ibuprofen is quite common. Rapid liquid chromatographic procedure proposed for analysis of Ibuprofen and Sitagliptin in pharmaceutical preparations using methanol: water (80:20 v/v) as a mobile phase, adjusting pH to 3.2 with UV detection at 275 nm. The advantages of this method include good and rapid separation, well resolved peaks, and only a small amount of sample is required for assay that display adequate precision. The method showed good linearity in the range of 231.25-1000 μg mL−1 for Ibuprofen and Sitagliptin with a correlation coefficient of 0.9999. The recovery of Ibuprofen and Sitagliptin was > 99.99% and > 99.96%, respectively. The proposed method may be used for the quantitative analysis of Ibuprofen and sitagliptin alone or in combination in bulk drugs and combined dosage formulations.

Author Biographies

Hina Rehman, Jinnah University for Women, Karachi

Faculty of Pharmacy

Safila Naveed, Jinnah University for Women, Karachi

Faculty of Pharmacy

Fatima Qamar, Jinnah University for Women, Karachi

Faculty of Pharmacy

Khan Usmanghani, Jinnah University for Women, Karachi

Faculty of Pharmacy

Ghulam Sarwar, Jinnah University for Women, Karachi

Faculty of Pharmacy

M. Tanweer Alam, Jinnah University for Women, Karachi

Faculty of Pharmacy

Amanullah Khan, Health Department Baluchistan

Drugs Testing laboratory Quetta,

Published
2015-12-12