Phytochemical Analysis and Tyrosinase Inhibitory Potential of Cassia absus (Seeds)

Saeed Ahmad; Tayyeba Rehman; Mariam Safdar; Ayesha Hassan

Saeed Ahmad The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Tayyeba Rehman The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Mariam Safdar The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Ayesha Hassan The Islamia University of Bahawalpur, Bahawalpur, Pakistan

Keywords: Anti-tyrosinase activity, Cassia absus, phytochemical analysis, tyrosinase, whitening

Abstract

Objective: To perform phytochemical analysis and investigation of the tyrosinase inhibitory activity of Cassia absus seeds.
Methods: Various extracts of Cassia absus seed were prepared such as ethanolic extract, n-hexane, chloroform, n-butanol and water fractions. Tyrosinase inhibitory activity was performed using Kim’s methodology. The extracts were also subjected to phytochemical analysis.
Result: Phytochemical analysis showed presence of alkaloids, tannins, flavonoids, glycosides and saponins. Tyrosinase inhibition assay was performed for all the four fractions, n-butanol showed high activity of tyrosinase inhibition (86%) followed by crude ethanol extract (75%) and n. hexane (72%) and water (62%) as compared to kojic acid (97%).
Conclusion: From all the results, we found that that phytochemicals present in C. absus seed extract may be responsible for the tyrosinase inhibition activity. The data revealed C. absus seed extracts/fractions could be an addition to medicinal cosmetics.