A Validated RP-HPLC Method for Determination of Rose Bengal in Bulk and Surgical Strips
A simple validated RP-HPLC method for determination of Rose Bengal (RB) Surgical strips which are used for staining of damaged cells of eye. The successful separation of RB was achieved on a Cromosil 100-5μm column (4.6 x 250mm with 5μm diameter) and detector of UV 262nm 1.0ml/min as a flow rate, and 20μl as an injector volume. The mobile phase of proposed method was methanol and phosphate Buffer of pH 8.0 in 50:50 (v/v) ratios. The linearity of method was determined in the range of 8-40ppm. The regression coefficient; LOD and LOQ were respectively 0.9987, 1ppm and 3ppm. It was validated according to the guidelines of ICH and USP.
Copyright (c) 2018 RADS Journal of Pharmacy and Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Submitting a manuscript to a journal implies that the work has not previously been published in any other journal, printed or online (save as an abstract or an academic thesis), and that it is not currently being considered for publication elsewhere.