A Validated RP-HPLC Method for Determination of Rose Bengal in Bulk and Surgical Strips

  • Abdul Mannan Ophth-Pharma (Pvt.) Ltd., Karachi, Pakistan
  • Akhter Jamal Khan Federal Urdu University of Arts, Science & Technology, Karachi, Pakistan
  • Mahwish Khan Ophth-Pharma (Pvt.) Ltd., Karachi, Pakistan
  • Ghulam Abass Ophth-Pharma (Pvt.) Ltd., Karachi, Pakistan
  • Sayyad Roohullah Ophth-Pharma (Pvt.) Ltd., Karachi, Pakistan
  • Zahid Khan Federal Urdu University of Arts, Science & Technology, Karachi, Pakistan
Keywords: RP-HPLC, ROSE BENGAL (RB), Validation

Abstract

A simple validated RP-HPLC method for determination of Rose Bengal (RB) Surgical strips which are used for staining of damaged cells of eye. The successful separation of RB was achieved on a Cromosil 100-5μm column (4.6 x 250mm with 5μm diameter) and detector of UV 262nm 1.0ml/min as a flow rate, and 20μl as an injector volume. The mobile phase of proposed method was methanol and phosphate Buffer of pH 8.0 in 50:50 (v/v) ratios. The linearity of method was determined in the range of 8-40ppm. The regression coefficient; LOD and LOQ were respectively 0.9987, 1ppm and 3ppm. It was validated according to the guidelines of ICH and USP.

Author Biographies

Akhter Jamal Khan, Federal Urdu University of Arts, Science & Technology, Karachi, Pakistan

Department of Pharmacognosy, 

Zahid Khan, Federal Urdu University of Arts, Science & Technology, Karachi, Pakistan

Department of Pharmacognosy, 

Published
2018-11-23
Issue
Vol 6 No 3 (2018): September
Section
Articles

Copyright (c) 2018 RADS Journal of Pharmacy and Pharmaceutical Sciences

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