Anti-nociceptive and Anti-inflammatory Effect of Monotheca buxifolia Leaves and Bark Extract: A Comparative Approach

Samiullah Burki; Mehjabeen .; Muhammad Arif Asghar; Zeba Gul Burki; Ahad Abdul Rehman

doi:10.37962/jpps.v9i2.520

Samiullah Burki Samiullah Burki1 Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University Karachi, Pakistan.
Mehjabeen . Federal Urdu University of Arts, Sciences and Technology, Karachi, Pakistan.
Muhammad Arif Asghar Jinnah Sindh Medical University
Zeba Gul Burki Federal Urdu University of Arts, Sciences and Technology, Karachi, Pakistan.
Ahad Abdul Rehman Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University Karachi, Pakistan.

DOI: https://doi.org/10.37962/jpps.v9i2.520

Keywords: Monotheca buxifolia, anti-nociceptive, anti-inflammatory, COX inhibition.

Abstract

Background: Inflammation plays an important role in various diseases with high prevalence within populations. Monotheca buxifolia has been long used in the folk medicine in urinary tract infections.

Objective: The aim of the study was to comparatively evaluate the anti-nociceptive and anti-inflammatory activities of M. buxifolia leaves and bark along with their fractions.

Methods: The anti-nociceptive activity of M. buxifolia was assessed via acetic acid induce writhing test. The anti-inflammatory activity was determined via formalin induce paw method, while in vitro anti-inflammatory and anti-nociceptive activity was carried out through COX-2 enzyme inhibition.

Results: The acetic acid induce anti-nociceptive results of M. buxifolia of bark were more significant (p<0.001) then leaves crude extract of M. buxifolia at 500 mg/kg. The chloroform fraction of both leaves and bark extract also depicts significant reduction 26.5±1.0 and 23.5±0.8 in number of writhes respectively at 500 mg/kg. The anti-nociceptive effect of M. buxifolia bark in paw licking was also significant. The paw licking duration with M. buxifolia leaves crude extract was 1.9±0.01 min at 500 mg/kg dose (p<0.001) in the early phase. The chloroform fraction also showed significant (p<0.001) reduction in paw licking duration. The COX-2 inhibitory assay of leaves extract of M. buxifolia exhibited 60.0±0.5% inhibition (p<0.001) at 1000 µg/ml. The chloroform fraction of M. buxifolia leaves at 1000 µg/ml showed 42.80 ±0.3 inhibition. The bark crude extract of M. buxifolia inhibited 63.83±0.73 % (p<0.001) COX-2 at 1000 µg/ml. The chloroform fraction of M. buxifolia bark at 1000 µg/ml showed 43.83 ±0.33 % inhibition. The results were also comparable with standard drug diclofenac sodium.

Conclusion: Based on promising anti-nociceptive and anti-inflammatory results, it is suggested that M. buxifolia should be a part of complementary and alternative medicine.