Assessment of Phenolic Contents, Cytotoxic, and Anti-Oxidant Potential of Euphorbia royleana

  • khuram Ashfaq Department of Pharmaceutical chemistry, Lahore Pharmacy College, Lahore
  • Muhammad Anees-ur-Rehman Ruth Pfau College of nutritional sciences, Lahore Medical and dental College, Lahore
  • Muhammad Tariq 3Department of pharmacology, Lahore Pharmacy College, Lahore
  • Nasir Ali Department of pharmaceutical chemistry, Lahore Pharmacy College, Lahore
Keywords: Euphorbia royleana, Total phenolics, Anti-oxidant, Cytotoxic.


Aim and Objective: The study was designed to assess the cytotoxic, phenolic contents and anti-oxidant potential of Euphorbia royleana.

Methods: The organic extracts were obtained by extraction of whole dried plant with n-hexane, dichloromethane and methanol. Pytochemical screening was carried out by using standard methods. Total phenolics contents were measured by using the Folin–CioEcalteu reagent method. Cytotoxic action was evaluated by brine shrimp lethality bioassay. DPPH radical assay was employed for anti-oxidant activity.

Results: Phytochemical investigations confirmed the presence of anthraquinones, saponins, cardiac glycosides and flavonoids. Highest phenolic content (276.41 ± 64 mg GAE/g dry extract wt) was observed in methanol extract. Significant antioxidant potential was exhibited by methanol extract by means of 78.8% inhibition with IC50 value of 35.71µg/ml. In brine shrimp lethality bioassay, methanol extract demonstrated prominent cytotoxicity at highest level of dose with LD50 471.05.

Conclusion: The study concluded that Euphorbia royleana contains significant antioxidant and cytotoxic potential. Further investigation is suggested for isolation of secondary metabolites responsible for reported biological activities.