Development and Validation of RP HPLC Method for Simultaneous Determination of Cefoperazone sodium and Sulbactam sodium in Dry Injection

  • Ahmed Khan Department of Chemistry, Lahore Garrison University, Lahore-Pakistan
  • Shabbir Hussain Department of Chemistry Khwaja Fareed University of Engineering and Technology Rahim Yar Khan, Pakistan
  • Muhammad Amjad Department of Chemistry, Lahore Garrison University, Lahore-Pakistan
  • Qamar Islam Department of Chemistry, Lahore Garrison University, Lahore-Pakistan
Keywords: RP-HPLC, Validation, Method, Development

Abstract

Background: Various analytical techniques are attempted for the qualitative and quantitative study of Cefoperazone sodium and Sulbactam sodium in different pharmaceutical dosage forms. These analytical techniques are not, easy, cost effective, eco-friendly and are so much laborious.

Objective: The main objectives of the study was to develop a simple, green, cost-effective, reproducible and precise isocratic RP-HPLC method for Cefoperazone Sodium and Sulbactam Sodium quantitative and qualitative studies in pure and dry injection powder form in compliance with ICH- Guidelines Q2 (R1).

Methodology: The chromatographic separation was carried out on a reversed phase C18 (250mm x 4.6 mm) 5 µm in an isocratic mode using tetrabutylammonium hydroxide solution and acetonitrile (8:3) as mobile phase adjusted pH 4.0 with dilute phosphoric acid and maintained flow rate 1.0 ml/minute at 220 nm. The retention time of Sulbactam sodium and Cefoparazone sodium was 7.4 min and 16.7 min respectively. All HPLC injection volume was 20µl in analysis.

Result:   The chromatographic separation was carried out on a reversed phase C18 (250mm x 4.6 mm) 5 µm in an isocratic mode using tetrabutylammonium hydroxide solution and acetonitrile (8:3) as mobile phase adjusted pH 4.0 with dilute phosphoric acid and maintained flow rate 1.0 ml/minute the retention time of Sulbactam sodium and Cefoparazone sodium was 7.4 min and 16.7 min respectively. All HPLC injection volume was 20µl and the retention time for Cefoperazone sodium and Sulbactam sodium was noted about 7.4 min and 16.7 min respectively.

Conclusion: A simple, precise, accurate and cost effective method has been developed by using RP-HPLC. The available method is simple and reliable to determine the Cefoperazone sodium and Sulbactam sodium in different pharmaceutical formulations and in their pure forms.

Published
2022-12-31