Anti-Motility and Anti-Diarrheal Activities of Lippia Nodiflora and Withania Somnifera Against Castor Oil and Magnesium Sulphate-Induced Diarrhea in Mice

Muhammad Shoaib Akhtar; Abdul Malik; Haroon Arshad; Sadaf Aziz; Tahira Tabassum; Muhammad Zeeshan Ali; Malik Hassan Mehmood; Qaisar Mahmood; Muhammad Riaz

doi:10.37962/jpps.v10i4.674

Muhammad Shoaib Akhtar Department of Pharmacology, College of Pharmacy, University of Sargodha, Sargodha-40100, Pakistan
Abdul Malik Department of Pharmacology, College of Pharmacy, University of Sargodha, Sargodha-40100, Pakistan
Haroon Arshad Health Department, Government of the Punjab, Lahore-54000, Pakistan
Sadaf Aziz Department of Pharmacology, College of Pharmacy, University of Sargodha, Sargodha-40100, Pakistan
Tahira Tabassum Department of Pathology, Sargodha Medical College, University of Sargodha-40100, Pakistan
Muhammad Zeeshan Ali Health Department, Government of the Punjab, Lahore-54000, Pakistan
Malik Hassan Mehmood Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad
Qaisar Mahmood Mukabir College of Pharmacy, Gujrat-50700, Pakistan
Muhammad Riaz Department of Allied Health Sciences, Sargodha Medical College, University of Sargodha, Sargodha, 40100, Pakistan.

DOI: https://doi.org/10.37962/jpps.v10i4.674

Keywords: Lippia nodiflora, Withania somnifera, anti-motility, anti-diarrheal, flavonoids and alkaloids

Abstract

Objective: The ongoing exploration is planned to narrate anti-motility and anti-diarrheal potential of L. nodiflora (LN.Cr) and W. somnifera (WS.Cr) hydro-alcoholic extract and their flavonoids and alkaloids rich fractions in case of castor oil and MgSO₄-mediated diarrhea in mice.

Methodology: Locally collected whole plant of Lippia nodiflora and roots of Withania somnifera were dried, pulverized and soaked into aqueous methanol to get hydro-alcoholic extracts. Alkaloids and flavonoids were separated from dried sample of each plant.

Result: L. nodiflora and W. somnifera showed significant inhibition (56.2 and 52%) of intestinal transport of charcoal like atropine (63%) and intestinal fluid accumulation (68 and 66%) in mice, respectively in dose-dependent manner with dosage range at 100-300 mg/kg. LN.Cr and WS.Cr showed dose-dependent safety in case of castor oil (67% and 63.3%) and magnesium sulphate-mediated diarrhea (69.2 % & 67%) at the highest tested dose of 300 and 200mg/kg, respectively in mice, like loperamide (73%). Isolated alkaloids and flavonoids also brought significant blockage of charcoal meal transit and safety against castor oil-mediated diarrhea.

Conclusion: This work suggested that L. nodiflora and W. somnifera extracts and their isolated alkaloids and flavonoids showed antidiarrheal activity by inhibiting intestinal motility, secretion of fluid and possibly inhibition of autacoids and prostaglandin. Antidiarrheal effects of flavonoids were more pronounced than alkaloids and crude extracts of both plants.