The Influence and Mechanism of Benzodihydropyran Benzene and Dihydro Pyran Derivatives xy2004 Effect on Breast Cancer Cell Proliferation and its Mechanism Ramification
Objective To investigate the effects of xy2004 on the proliferation of MCF-7 cells and its mechanism. Methods Effects of xy2004 on the proliferation of MCF-7 cells was determined by MTT assay. Cell apoptosis was examined by flow cytometry. Apoptosis proteins were examined by western blot Competitive estrogen-receptor binding assay was used to investigate the affinity of xy2004 ER. Results xy2004 induced cell proliferation of MCF-7cells in low concentrations and inhibited cell proliferation in high concentrations. Antiestrogen tamoxifen eliminated the stimulation of proliferation of MCF-7 induced by xy2004. The relative binding affinity of xy9906 for ERα and ERβ showed an IC50 of 7.38×10-5M and 4.12×10-7M respectively. After MCF-7 cells were treated with xy2004 in high concentration, Bcl-2 protein expressions were reduced and Bax protein expressions were increased. Conclusion xy2004 directly stimulates cell proliferation of MCF-7 cells through ligand-receptor binding in low concentrations and inhibited cell proliferation in high concentrations through regulating the apoptosis proteins.