Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Intellan Capsule

  • Aqib Zahoor Herbion Pakistan (Pvt.) Limited, Karachi
  • Hira Munir Herbion Pakistan (Pvt.) Limited, Karachi
  • Shafiquat Hussain Herbion Pakistan (Pvt.) Ltd., Karachi, Pakistan
  • Kashifa Khanum Herbion Pakistan (Pvt.) Ltd., Karachi, Pakistan
  • Safila Naveed Jinnah University for Women, Karachi
  • Khan Usmanghani Jinnah University for Women, Karachi
Keywords: Vinpocetine, formulation, RP-HPLC

Abstract

Simple, precise, cost-effective and accurate high performance liquid chromatographic method for the determination of vinpocetine in API (active pharmaceutical ingredient) and Intellan Capsule formulation has been developed and validated. Chromatography was carried out at ambient temperature on a prepacked Purospher Star, (5 mm, 250 x 4.6 mm) and Hibar 250- 4.6 columns with the isocratic mobile phase of acetonitrile: Ammonium acetate (55:45 v/v) adjusting pH to 2.8. The UV detection was carried at 280 nm. The results obtained showed good agreement with the declared contents. Vinpocetine detected in less than 25 mins with good resolution and minimal tailing and without interference of excepients. The method was linear in the range of 5–100 μgmL-1 with a correlation co-efficient 0.999 (inter and intraday CV​

Author Biographies

Safila Naveed, Jinnah University for Women, Karachi

Faculty of Pharmacy

Khan Usmanghani, Jinnah University for Women, Karachi

Faculty of Pharmacy

Published
2016-01-17
Issue
Vol 4 No 1 (2016): Volume 4 (1) 2016
Section
Articles

Creative Commons License

Submitting a manuscript to a journal implies that the work has not previously been published in any other journal, printed or online (save as an abstract or an academic thesis), and that it is not currently being considered for publication elsewhere.